Download e-book for kindle: Amino Acid Receptor Research by Benjamin F. Paley, Tomas E. Warfield

By Benjamin F. Paley, Tomas E. Warfield

ISBN-10: 1604562838

ISBN-13: 9781604562835

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Representative immunofluorescent images of HEK293 cells that were subjected to electroporation without DNA (left column, a, c and e) and positively transfected cells by cDNA encoding the NR1a, NR2B subunit and CD8 (right column, b, d and f). From top to bottom, cells were treated with a primary antibody against the NR1a (b), the extracellular domain of NR2B (d) and the Cterminal domain of NR2B (f). Bar : 10 µM. The percentage of successfully transfected cells was further verified by immunofluorescence staining.

There is also the possibility of culturing retinal cells conserving its laminated pattern of cell bodies and processes using small pieces of retinal tissue, the explant culture (Zhang et al, 2003; Yamagata and Sanes, 1995), or aggregate cultures, also called retinospheroid cultures (Layer and Willbold, 1993). The dissection 20 Roberto Paes-de-Carvalho, Karin da Costa Calaza, Marcelo Cossenza et al. procedure is reliable and does not cause extensive damage to cells, since the chick retina is a non-vascularized tissue and conserve a high degree of glicolytic metabolism.

1990). Apparent desensitization of NMDA responses in Xenopus oocytes involves calcium-dependent chloride current. , 4, 53-60. Ma, Z. , Wahle, P. & Hollmann, M. (2007). Quantitative analysis of cotransfection efficiencies in studies of ionotropic glutamate receptor complexes. , 85, 99-115. Marchetti, C. & Gavazzo, P. (2003). Subunit-dependent effects of nickel on NMDA receptor channels. Brain Res Mol Brain Res, 117, 139-44. Marchetti, C. & Gavazzo, P. (2005). NMDA receptors as targets of heavy metal interaction and toxicity.

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Amino Acid Receptor Research by Benjamin F. Paley, Tomas E. Warfield


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