Advances in Genetics, Vol. 23 - download pdf or read online

By E.W. Caspari, John G. Scandalios (Eds.)

ISBN-10: 0120176238

ISBN-13: 9780120176236

This quantity in a sequence on genetics, emphasizes the range of genetic reports. Articles hide the filamentous fungus neurospora, biogenesis of yeast ribosomes, evolutionary genetics of fish, drosophila transposable components and the dropophila gene zeste.

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Notes: The progressive enhancement of radiation killing by caffeine during repair is illustrated by the comparison between the curves ( 0 )and (0). The presence of caffeine after irradiation always results in an increase in the radiation-induced lethality, (0) versus (0). A delay in the treatment with caffeine suppresses the killing potentiation. The data have been interpreted as an inhibition by caffeine of a n early step in the repair of y-ray-induced DNA injuries. From Gentner and Werner (1975).

In G, cells DNA synthesis must first occur in order to obtain the necessary second DNA copy. In S. , 1978). In wild-type G, cells, the DNA synthesis occurs immediately after the transfer of the unirradiated cells into complete medium and the genome is duplicated after 4 hr, although cell division does not resume before 6 hr. , 1978). b. Caffeine as a Tool for Studying Recombinational Repair. It has been observed that only one of the two UV damage repair pathways in S. pombe is sensitive to caffeine (Clarke, 1968; Schupbach, 1971; Fabre, 1971).

Postirradiation caffeine sensitivity to y rays only: rad 7, rad 12, rad 14. Group 3: Mutants sensitive to y rays only. Postirradiation caffeine sensitivity to both radiations: rad 21, rad 22. Double mutants have been constructed (Nasim and Smith, 1974) and are listed in Table 3. In spite of the attempt by Nasim and Smith (1974) no triple mutant has been found so far that would confirm the existence of the third repair pathway as suggested by Gentner (1977). Such RECOVERY AND REPAIR IN Schizosaccharomyces 33 mutants should be sought in yeast strains already mutated in both pathways I and 111,using increased sensitivity for caffeine following yirradiation.

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Advances in Genetics, Vol. 23 by E.W. Caspari, John G. Scandalios (Eds.)


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