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3 Preparation of SMC and Scale-Up Strategies . . . . . . 2 Synthesis of the SMC . . . . . . . . . . . . . 62 Preparation of Large Scale SMC . . . . . . . . . . 63 4 Characteristics and Application of SMC in the Liquid Chromatography of Biomolecules . . . . . . . . . 1 Characteristics of the SMC . . . . . . . . . . . 69 Application of SMC for Liquid Chromatography of Biomolecules . 72 Plasmid DNA Purification . . . . . . . . . . . 75 5 Other Applications of SMC .

28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. F. Svec Schmeer K, Behnke B, Bayer E (1995) Anal Chem 67:3656 Boughtflower RJ, Underwood T, Maddin J (1995) Chromatographia 41:398 Dittmann MM, Wienand K, Bek F, Rozing GP (1995) LC-GC 13:800 Crego AL, Gonzalez A, Marina ML (1996) Crit Revs Anal Chem 26:261 Colon LA, Reynolds KJ, Maldonado RA, Fermier A (1997) Electrophoresis 17:2162 Robson MM, Cikalo MG, Myers P, Euerby MR, Bartle KD (1997) J Microcol Sep 9:357 Altria KD, Smith NW, Turnbull CH (1997) Chromatographia 46:664 Rathore AS, Horvath C (1997) J Chromatogr A 781:185 Cikalo MG, Bartle KD, Robson MM, Myers P, Euerby MR (1998) Analyst 123:87R Krull IS, Stevenson R, Mistry K, Schwarz ME (2000) Capillary electrochromatography and pressurized flow capillary electrochromatography: an introduction.

51 52 53 54 55 55 Advances in Biochemical Engineering/ Biotechnology, Vol. 76 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 2002 50 A. Strancar et al. 3 Preparation of SMC and Scale-Up Strategies . . . . . . 2 Synthesis of the SMC . . . . . . . . . . . . . 62 Preparation of Large Scale SMC . . . . . . . . . . 63 4 Characteristics and Application of SMC in the Liquid Chromatography of Biomolecules . . .

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